摘要
目的:研究单免疫球蛋白白介素1受体相关蛋白(SIGIRR)对脂多糖(LPS)刺激的肺泡上皮细胞核因子-κB信号通路的调节作用。方法:以LPS刺激人Ⅱ型肺泡上皮细胞A549。将含有SIGIRR cDNA全长的真核表达载体瞬时转染A549细胞,使SIGIRR在A549细胞过表达。用双萤光素酶报告系统检测LPS刺激后A549细胞NF-κB的转录活性,用ELISA法检测LPS刺激后细胞因子白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)以及白细胞介素-6(IL-6)的水平,对比转染与否对上述因子在A549细胞中释放水平的影响。结果:在A549细胞中,过表达SIGIRR可显著抑制NF-κB的转录活性,同时抑制LPS诱导的细胞因子IL-1β、TNF-α以及IL-6的表达。结论:SIGIRR过表达可以抑制LPS触发的肺泡上皮细胞中TLR4信号的转导,从而发挥减轻炎症反应、保护肺泡上皮细胞的作用。
AIM : To investigate the effect of single immunoglobin IL - 1 receptor related protein (SIGIRR) on damage of alveolar epithelial cells in acute lung injury induced by lipopolysaccharide. METHODS : The acute alveolar epithelial cell injury model was constructed by stimulation of A549 cells with LPS. In order to over - express SIGIRR, the A549 cells were transferred with eukaryotic expression vector containing full length SIGIRR cDNA. The transcriptional activity of NF - KB was measured by dual - luciferase reporter assay system. The concentrations of IL - 16, TNF - α and IL - 6 were detected by ELISA. The levels of these inflammatory factors between the transfected cells and untransfected cells were compared. RESULTS : The over - expression of SIGIRR inhibited the transcriptional activity of NF - κB. The increases in IL - 1β, TNF - α and IL - 6 concentrations in alveolar epithelial cells induced by LPS were observed. CONCLUSION: SIGIRR in alveolar epithelial cells inhibits TLR4 signals triggered by LPS and attenuates the inflammatory reactions in alveolar epithelial cells, which plays a protective role against the acute damage of the alveolar epithelial cells.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2010年第2期379-383,共5页
Chinese Journal of Pathophysiology
基金
军队"十一五"医药卫生科研资助项目(No.06MA206)