中国石竹组培苗玻璃化和成苗率若干影响因素的研究

On Reducing Vitrification In Pink Stem Apex Culture

本文就中国石竹茎尖组培苗玻璃化和成苗率的若干因素进行了研究．对外植体消毒方法进行了改进，其中采用0．1％的氯化汞溶液对防止中国石竹茎尖培养中玻璃苗的产生和提高成苗率有明显效果．在培养基配方中降低植物激素和糖的用量，增加琼脂的浓度，能有效防治玻璃化苗的产生．

In this paper we study some factors that contribute to the Vitrification in pink stem apex culture. We make improvement on the traditional methods to disinfect the materials. Specifically, we use 0.1% HgCl2 solution instead of 75 % alcohol and 1% NaCIO3. As a result, the vitrification is well under contrel. So that the survival rate of the stem apex in culture is increased dramatically. It is also discovered that culture media is an element that has to be taken into consideration in reducing the vitrification; formulations with less hormone, sugar, salt, and more. Agar are found to work more effectively.