山猪血清蛋白质多态性聚丙烯酰胺凝胶电泳分析研究

Polyacrylamide Gel Electrophoresis Analysis of Serum Protein in Shan Pig

运用聚丙烯酰胺凝胶电泳分析了山猪血清蛋白质生化位点多态性的分布.结果表明,前白蛋白Pa、白蛋白Alb、后白蛋白Po可分离出4～6条区带,转铁蛋白Tf分离出2～3条区带.白蛋白的平均分子量约为69000Da,转铁蛋白的平均分子量约为88000Da.用聚丙烯酰胺凝胶电泳分离血清蛋白质重复性较好,分辨较高,测定蛋白质的多态性和分子量的变化对分析猪的遗传特征、选育优良品种和诊断诸多疾病有重要意义.

The electmphoertograms of protein of serum in Shan pig were analysed by the method of polyacrylamide gel electrophoresis. The result showed that the serum proteins, such as Pa, albemoglobin （ Alb）, Po were separated into 4 - 6 zones, and Tf into 2 - 3 zones. The average molecular weight of albemo- globin（Alb） was about 69000 Da and Tf about 88000 Da. The separating of serum protein by polyacrylamide gel electrophoresis had fairly good repeated re- suits and resolution ratio. The polymorphism and molecular weight of percentage in protein had important meanings for the hereditary feature analysis,breed choose and diseases diagnasis of the pigs.