13种脊椎动物miRNA碱基组成分析

An Analysis on the Base Composition of miRNAs in Thirteen Vertebrate

miRNAs是当前基因表达调控领域的研究热点.本研究借助于Sanger实验室提供的miRNA数据库9.0,使用相关的生物信息学软件统计分析13种脊椎动物miRNA的四种碱基、GC和AU含量,所取得的miRNA一级结构特征信息对全面了解miRNA的基本生物学特性以及miRNA基因识别策略的设计都具有重要意义.结果表明,所分析的13种脊椎动物的miRNA序列中,腺嘌呤(A)含量除了非洲蛙与褐家兔及小家鼠之间在前体miRNA存在差异外,其余均未见显著差异,表明不同物种的miRNA中A含量基本相同,保持相对稳定;同样,各种生物之间,成熟miRNA的鸟嘌呤(G)含量也未见显著性差异;在不同脊椎动物之间,miRNA序列碱基组成上存在一些差异.

The miRNAs are a new class with about 20 -24 nts of small non-coding RNA presented extensively in the plant, nematodes and human cells. At present the study on miRNAs is hot in the field of current gene expression regulation. In this paper, the contents of A, U, G, C, GC and AU in the known miRNA sequences in thirteen vertebrate are analyzed. In order to determine the base contents of miRNA in vertebrate, miRNA database （ version 9.0） provided by Sanger＇s laboratory is employed and the contents of A,U,G,C,GC and AU in the known miRNAs sequences found in thirteen kinds of vertebrate are determined by means of bioinformatics softwares. The results show that there are no obvious difference in almost all the above speeies＇contents of A and G in miRNA, and the content of AU is higher than that of GC in any sequence studied. Furthermore, no siginificant difference are found in the contents of the four kinds of base composition between the pre-miRNA and mat-miRNA in the same species, however, C in Drosophila melanogaster is excluded.