围绝经期潮热模型大鼠的尾温变化及意义探讨

Changes of Tail Skin Temperature in Ovariectomized Rats in Relation to Menopausal Hot Flush

目的建立大鼠围绝经期潮热动物模型。方法将大鼠随机分为实验组和对照组,实验组行双侧卵巢切除术,对照组行假手术,术前第1周至术后第9周,每周连续测量鼠尾皮肤温度和肛温。结果在去卵巢后1～4周,与对照组大鼠(25.9±0.1)℃比较,实验组大鼠的尾温出现明显升高(26.8±0.4)℃(P<0.05);在去卵巢后5～9周,与对照组大鼠(25.6±0.2)℃比较,实验组大鼠的尾温又明显下降(24.5±0.2)℃(P<0.01),提高环境温度后,实验组大鼠的尾温较对照组明显升高(P<0.01)。结论卵巢功能减退和消失使大鼠立即出现尾温升高,当环境温度升高时也会促发尾部频繁散热,这一特征与围绝经期妇女的潮热症状相似,可作为围绝经期潮热的动物研究模型。

Objective To found a rat model for menopausal hot flush. Methods Rats were randomly allocated into two groups. One group （OVX） was bilaterally ovariectomized, and the other group （Sham） underwent a sham operation. Both tail skin temperature（TST）and rectal temperature（RT）were measured,and their mean values,the TST and the RT,were calculated every week from 1 week before to 9 week after the ovariectomy. Results The TST in OVX rats（26.8±0.4）℃ was significantly（P〈0.05）elevated from 1 to 4 week after the ovariectomy compared with that in Sham rats（25.9±0.1） ℃ ,whereas the TST in OVX rats（24.5±0.2）℃ was significantly（P〈 0. 01） declined from 5 to 9 week compared with that in sham-operated rats（25.6±0.2）℃. The TST in OVX rats was significantly（P〈0.01） increased after elevation of environment temperature compared with that in sham rats. Conclusion The elevation of TST in OVX rats was exhibited soon after the estrogen removal with time. These characteristics are similar to the symptoms of menopausal hot flush in women. Thus the elevation of TST in OVX rats is a good model for the study of menopausal hot flush.