不同免疫途径对蛋用鸡分泌型免疫球蛋白A细胞分布的影响

Effect of Immune Ways on the Distribution of Immunoglobulin A secreting cells in layers

用鸡新城疫弱毒疫苗通过饮水、滴鼻和肌肉注射免疫蛋用鸡，以葡萄球菌Ａ蛋白辣根过氧化酶和兔抗鸡分泌型免疫球蛋白Ａ（ＳＩｇＡ）间接组织化学染色，显示消化道相关淋巴组织（ＧＡＬＴ）和呼吸道相关淋巴组织（ＲＡＬＴ）中ＳＩｇＡ细胞的生成及分布。结果表明，滴鼻和饮水免疫组蛋用鸡ＧＡＬＴ和ＲＡＬＴ中产生ＳＩｇＡ细胞较早且多，并于首免后２８ｄ达最高峰，一直持续到首免后５６ｄ。肌肉注射组蛋用鸡ＳＩｇＡ细胞在各相应部位数量较少，且持续时间较短。

Layers were immunized with newcastle disease attenuated vaccine by oral, nasal and muscle inoculation to observe the immunoglobulin A secreting cells (SIgA) in gut associated lymphoid tissue (GALT) and respiratory tract associated lymphoid tissue (RALT), by means of staphyloccocus protein A horseradish peroxidase and rabbit anti chicken immunoglobulin A secreting (SIgA) cells indirect histochemical staining method. The results showed that the SIgA cells yielded more and earlier in oral and nasal immune treatment than in muscle inoculation and reached the peak value on the 28th day after the first vaccination which lasted 28 days.