罗格列酮对糖尿病大鼠残存胰岛表达肝细胞生长因子的影响

The effect of rosiglitazone on the expression of HGF in the survival pancreatic islet of diabetic rats

目的观察罗格列酮对实验性1型糖尿病大鼠残存胰岛表达肝细胞生长因子(HGF)的影响。方法 SD大鼠随机分为3组,正常对照(Con)组24只,糖尿病(DM)组24只,罗格列酮干预(RGZ)组24只。于应用罗格列酮后5个时间段分别测定各组FBG、FIns 。免疫组化法检测胰岛中Ins及HGF的表达,并进行形态学量化分析。结果与DM组相比,RGZ组经罗格列酮干预后2周,血清胰岛素水平逐渐上升;血糖水平逐渐下降;胰腺相对β细胞量明显增多;胰岛HGF表达水平提高(P均<0.05)。RGZ组胰岛HGF表达水平与胰腺相对β细胞量(r=0.766,P<0.05)及血清胰岛素水平(r=0.740,P<0.05)呈正相关。结论罗格列酮能增加实验性1型糖尿病SD大鼠β细胞数量,其机制可能与上调胰岛HGF的表达有关。

Objective To explore the effect of rosiglitazone on the expression of HGF in the survival pancreatic islet of diabetic rats. Methods Sprague-Dawley rats were randomly assigned as control group （n= 2,1） and diabetic control group（DM group, n= 24） and rosiglitazone（5mg·kg^-1 · d^-1） treated diabetic group （RGZ group, n = 24）. One-, two-, four-, seven-, and ten weeks after rosiglitazone treatment, fasting blood samples were collected for the assessment of FBG, insulin（Ins）. The expression levels of INS and HGF in pancreatic islet were detected by immunohistochemistry and the relative amount of islet cells were evaluated. Results In RGZ group, the decreased levels of blood Ins were increased over time （P〈0. 05）, and the increased FBG levels were decreased gradually and the relative quantity of β-cell was raised significantly（P〈0. 05）. The expression of HGF in pancreatic islet was significantly higher in RGZ group than in DM group two weeks after the treatment （P〈0.05） ,and the expression levels of HGF were paralleled to FBG（r=0. 740,P〈0.05） and relative β-cell volume（r=0. 766,P〈0. 05）. Conclusions Rosiglitazone can increase beta cell mass in diabetic rats and the mechanism may be related with the upregulated expression of HGF in pancreatic islet.