山杨抗锈病细胞系的筛选与新种质的培育

Cultivation and Selection of the Rust-Resistant Clones of Populus davidiana

采用生长快、材质优、抗性强的山杨优良品系为材料，通过组织培养获得细胞愈伤组织，再以锈孢子菌为活体病原菌进行接种，经多代反复筛选，获得出Ｐ２１５－３、Ｐ３０６－９、Ｐ３１８－１三个抗锈病细胞系，并诱导其形成植株。经３个世代（细胞团）、３个地区连续３年抗性鉴定，确认以上３个山杨抗锈病优良新种质表现出稳定的抗性，发病指数平均为２．９％，其中Ｐ３１８－１为２．５％，而对照Ｐ２０３发病指数平均为１６．６％。

Using the fast-growing, good wood and resistant clone of P.Davidiana, the callus was produced by tissue culture and then inoculated with aecidiospore and three rust-resistant clones(P 215-3,P 306-9,P 318-1) were selected after generations, and the plantlets were producted. They were confirmed as the rust-resistant clone, tested in 3 sites for 3 years and showed stable rust resistance. They also have aver susceptible percentage of 2 9%, such as P 318-1 is 2 5% but CK is 16 6%.