摘要
根据克隆到的ISLGa片段与ISLGb片段核苷酸序列之间的差异,发展了1个SCAR分子标记ISLGb(ISLGb-L+PS15),其引物只在具有ISLGb基因型的3个材料中扩增,并且均得到了大小为1265bp的单一亮带。通过与已有的相关分子标记整合得到了1套SCAR分子标记体系。包括2个多重PCR分子标记:①ISLG(PS5+PS15)和SP110(SP11-L+SP11-R')用于鉴别ISLG和IISP11b;②ISLGb(ISLGb-L+PS15)与SP11a(SP11-L+SP11-R)用于鉴别ISLGb和IISP11。还包括1个单PCR分子标记:SRKa(S40-5+S60-2)用于鉴别IISRKa。
According to differences between the nucleotide sequences of two fragmnts ,ISLGa and ISLCb ,a SCAR molecular marker named as ISLGb （ISLGb-L＋PS15） was developed and only amplified a band of 1 265 bp in three ISLGb genotype materials. After integrating the relevant molecular markers, a set of SCAR molecular markers was acquired, including two multiplex PCR molecular markers ： ①ISLG （PS5＋PS15） and SP110 （SP11-L＋ SP11-R＇） were used to identify ISLG and IISP11b ;②ISLGb （ISLGb-L＋PS15） and SP1 la（SP11-L＋SP11-R） were used to identify ISLGb and IISP11. Meanwhile ,a single PCR molecular marker,SRKa （S40-5＋S60-2） was used to identify IISPKa.
出处
《现代农业科技》
2010年第1期29-30,共2页
Modern Agricultural Science and Technology
