摘要
用高效液相色谱法和酶解的方法检测了银杏叶片磷脂酰甘油(PG)脂肪酸的分子种组成和位置分布,确定银杏叶片PG主要分子种的脂肪酸组成(sn1/sn2)是18∶3/16∶1(3t),18∶3/16∶0,18∶2/16∶1(3t),18∶2/16∶0,18∶1/16∶1(3t),16∶0/16∶1(3t),18∶1/18∶1,18∶1/16∶0和16∶0/16∶0。银杏叶片PG脂肪酸组成和位置分布的分析结果表明,C18脂肪酸主要位于sn1位,16∶1(3t)只分布于sn2位,16∶0在sn1位和sn2位上均有发现。sn1位上的不饱和度∑u大于sn2位上的∑u。
The composition of molecular species and the positional distribution of fatty acids of phosphatidylglycerol (PG) isolated from Ginkgo leaf were examined and analyzed by high performance liquid chromatography (HPLC) and enzymic hydrolysis. The fatty acid compositions (sn 1/sn 2) of the identified major molecular species were 18∶3/16∶1(3t),18∶3/16∶0,18∶2/16∶1(3t),18∶2/16∶0,18∶1/16∶1(3t),16∶0/16∶1(3t),18∶1/18∶1,18∶1/16∶0 and 16∶0/16∶0. The results showed that C 18 fatty acids were principally localized at the sn 1 position, the component 16∶1 (3t) exclusively occupied the sn 2 position and 16∶0 was found at both the sn 1 position and the sn 2 positon. The degree of unsaturation at the sn 1 postion was higher than that at the sn 2 positon.
出处
《植物学通报》
CAS
CSCD
1998年第5期50-55,共6页
Chinese Bulletin of Botany
基金
国家自然科学基金
关键词
银杏叶
磷脂酰甘油
分子种组成
脂肪酸
抗寒性
Ginkgo, Phosphatidylglycerol, Molecular species, Fatty acids, Positional distribution