花生种子萌发过程中胚轴多胺氧化酶的活性变化

THE CHANGES OF POLYAMINE OXIDASE ACTIVITY IN EMBRYONIC AXES DURING PEANUT SEED GERMINATION

研究花生（Ａｒａｃｈｉｓｈｙｐｏｇａｅａ）种子萌发过程中胚轴多胺氧化酶（ＰＡＯ）的活性变化及其与种子萌发的关系表明：胚轴中的ＰＡＯ活性是在种子萌发过程中逐渐形成的，而黑暗条件更有利于该酶的活性形成；放线菌素Ｄ（１０ｍｇ／Ｌ）、环己酰亚胺（１０ｍｇ／Ｌ）处理对种子萌发的抑制率分别为２６．３％和８７．３％，对胚轴ＰＡＯ活性的抑制率分别为４１．１％和９４．０％，显示胚轴中的ＰＡＯ很可能参与花生种子的萌发过程，且其ｍＲＮＡ在种子发育过程中已合成并贮存于种子中，萌发时ＰＡＯ活性的出现主要是由于这些ｍＲＮＡ转译合成了ＰＡＯ。

The activity of polyamine oxidase in embryonic axes during peanut ( Arachis hypogaea )seed germination was investigated. Peanut seeds were soaked for 12 h and allowed to germinate at 30 ℃ in moistened vermiculite. The PAO activity did not appear within the first 12 h of germination, but occurred when the germination time was 24 h and increased from then on. The PAO activity was higher when the peanut seeds germinated in the dark than in the light (1600 Lx). When the peanut seeds were treated with 10 mg/L actinomycin D (Act D), a RNA sunthesis inhibitor or cycloheximide (CHM), a protein synthesis inhibitor for 96 h, the inhibitions of germination and PAO activity were 26.3% and 41.1% by Act D or 87.3% and 94.0% by CHM. All of these results suggested that PAO was involved in the peanut seed germination.