HPLC法测定人血清中利奈唑胺浓度及其临床应用

HPLC determination and clinical application of linezolid concentration in human serum

目的:建立高效液相色谱法快速测定人血清中利奈唑胺浓度,并用于危重患者治疗药物监测。方法:色谱柱:Hypersil ODS柱;流动相:乙腈-水(23:77),调整pH至5.0;流速:1.0mL.min-1;紫外检测波长为254nm。应用已建立的方法对9例危重患者进行利奈唑胺治疗药物监测,并合理制定个体化给药方案。结果:利奈唑胺在0.31～20.00mg.L-1浓度范围内线性关系良好(r=0.9996),最低定量限为0.31mg.L-1,绝对回收率为47.2%,相对回收率为102.1%,日内、日间精密度的RSD分别<4%和<3.5%。另外,所监测9例危重患者中,有4例患者出现利奈唑胺谷浓度小于最低抑菌浓度的情况。结论:HPLC法简单、快速、准确、灵敏、重现性好,可用于临床利奈唑胺的浓度监测。

Objective：To establish a method of determining serum linezolid in human by HPLC,and use the method for therapeutic drug monitoring in critically ill patients.Methods：Separation was carried out on a Hypersil ODS column,the mobile phase consisted of acetonitrile-water （23∶77）,adjust the pH to 5.0 at the flow rate of 1.0 mL/min-1.UV detection wavelength was 254 nm.The method has been used for nine critically ill patients for therapeutic drug monitoring,and individual dosage regimen of linezolid.Results：A good linearity was demonstrated between 0.31-20.00 mg/L-1 （r=0.9996）,the lowest limit of quantification of 0.31 mg/L-1.The absolute recovery was 47.2%,and the relative recovery was 102.1%.The intra-day and inter-day deviation was showed by RSD〈4%,〈3.5%,respectively.In addition,the monitoring of nine critically ill patients,there are four patients with linezolid trough concentrations less than the minimum inhibitory concentrations.Conclusion：The method is simple,rapid,accurate,sensitive,reproducible,and validated for determination of serum linezolid in clinical practice.