白花蛇舌草超高效液相色谱特征图谱研究Ⅱ——强极性组分特征图谱研究

Specific chromatograms analysis of Hedyotis diffusa Willd. by ultra-performance liquid chromatography (UPLC) Ⅱ——Studies on strong hydrophilic components

目的:发展中药白花蛇舌草强极性组分的超高效液相色谱(UPLC)特征图谱,进一步加强中药材的质量控制。方法:建立不同产地白花蛇舌草及其伪品水线草中强极性组分的UPLC亲水模式分析方法,并对其特征图谱进行相似度考察和主成分分析。结果:强极性化合物特征图谱增加了中药材特征图谱的信息量,该组分特征图谱同非强极性组分特征图谱一样具有地域性。结论:强极性组分特征图谱和非强极性组分特征图谱共同应用,有利于提高中药材质量控制的水平。

Objective：To develop the ultra-performance liquid chromatography （UPLC） specific chromatograms basing on strong hydrophilic components in Hedyotis diffusa Willd.in hydrophilic interaction chromatography （HILIC） mode,and to investigate the more advantages when specific chromatograms analyzed of strong and mild hydrophilic components were together used for the quality control of Hedyotis diffusa than that only with the total extract.Methods：An UPLC method was established in hydrophilic interaction chromatography mode for the analysis of the strong hydrophilic components in Hedyotis diffusa and its substitutes from different places.The UPLC specific chromatograms was analyzed by the similarity and principle component analysis methods.Results：The specific chromatograms of strong hydrophilic components greatly increased the specific chromatograms information.It can be applied to distinguish Hedyotis diffusa from different places and its substitutes.Conclusion：The combinative specific chromatograms analyses of strong hydrophilic components with mild hydrophilic components can obviously improve the quality control level of traditional Chinese medicines.