摘要
目的:建立了一种简单、灵敏的酶催化动力学光度法测定盐酸多巴胺的新方法。方法:在pH9.8的NH3-NH4Cl的缓冲溶液中,利用盐酸多巴胺对牛血红蛋白(Hemoglobin,Hb)模拟酶催化体系的抑制作用,研究了该抑制反应的最佳实验条件及动力学行为。结果:测定的线性范围为1.1×10-7~1.1×10-5mol.L-1,检出限为6.1×10-8mol.L-1。对浓度为5.0×10-6mol.L-1的盐酸多巴胺进行11次平行测定,其相对标准偏差为4.5(。结论:该方法简单,快速,灵敏,可用于盐酸多巴胺注射液中盐酸多巴胺含量的测定。
Objective:To develop a high sensitive and simple spectrophotometric method for the determination of dopamine hydrochloride.Methods:The determination of dopamine hydrochloride based on the inhibition of hemoglobin for the oxidation of H2O2 with acid chrome blue K(ACBK) in the NH3-NH4Cl buffer.The percentage inhibition (I﹪) of system is calculated under the optimal experimental condition.Results:The calibration curve is linear in the range of 1.1×10-7-1.1×10-5 mol/L-1,with the detection limit of 6.1×10-8 mol/L-1.The relative standard deviation of this method is 4.5% at 5.0×10-6 mol/L-1 for 11 determination.Conclusion:This method is simple,rapid and sensitive which is suitable for the determination of dopamine hydrochloride in dopamine hydrochloride injection with satisfactory results.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2010年第2期314-316,共3页
Chinese Journal of Pharmaceutical Analysis
基金
河南省高校科技创新人才支持计划资助(No.2009HASTIT034)
河南省教育厅自然科学基金资助项目(No.2008A15002)
关键词
盐酸多巴胺
酶催化动力学光度法
血红蛋白
dopamine hydrochloride
enzymatic catalytic spectrophotometry
hemoglobin