RP-HPLC法测定月腺大戟根中2种三萜类成分的含量

RP-HPLC determination of two triterpenoids in roots of Euphorbia ebracteolata Hayata

目的:对月腺大戟根中的2种主要三萜类成分β-香树脂醇乙酸酯(化合物Ⅰ)和24-亚甲基环阿尔廷醇(化合物Ⅱ)进行含量测定。方法:采用反相高效液相色谱法,使用Agilent HC-C8柱(150 mm×4.6 mm,5μm),流动相为乙腈-水(85∶15),流速1.0 mL.min-1,检测波长205 nm,柱温25℃。结果:化合物Ⅰ和Ⅱ进样量均在0.1～5.0μg范围内有良好的线性关系,相关系数均在0.9998以上(n=6);平均回收率(n=6)分别为99.3%和96.9%,RSD分别为1.9%和0.86%。结论:本方法能够准确测定月腺大戟根中2种主要三萜类成分的含量。

Objective：To determine two triterpenoids,luteolin β-amyrin acetate（compound Ⅰ） and 24-methylene-cycloartanol（compound Ⅱ）,in roots of Euphorbia ebracteolata Hayata.Methods：Separation was carried out on Agilent HC-C8 column（150 mm×4.6 mm,5 μm）.The mobile phase was acetonitrile-water（85∶15）at a flow rate of 1.0 mL·min-1 at 25 ℃,and the detector was set at 205 nm.Results：The linearity was in the range of 0.1-5.0 μg for compoundsⅠand Ⅱ,and the correlation coefficients were more than 0.9998（n=6）.The average recoveries（n=6）for compound Ⅰand Ⅱ were 99.3%（RSD=1.9%）and 96.9%（RSD=0.86%） respectively.Conclusion：This method is accurate,reliable and sensitive for determination of two triterpenoids in Euphorbia ebracteolata Hayata.