摘要
目的:运用高效液相色谱法分析3个核苷类即尿苷、鸟苷、腺苷以及腺嘌呤在6种常用补益中药人参、黄芪、熟地黄、当归、麦冬和肉苁蓉中的含量。方法:采用Zorbax SB-Aq(250 mm×4.6 mm,5μm);以0.05%甲酸水溶液-乙腈为流动相梯度洗脱,流速0.5 mL.min-1;检测波长260 nm;柱温30℃。结果:尿苷、腺嘌呤、鸟苷和腺苷分别在0.30~77.32μg.mL-1(r=1.000)、0.23~58.29μg.mL-1(r=0.9998)、0.35~90.73μg.mL-1(r=1.000)和0.61~157.07μg.mL-1(r=1.000)范围内线性关系良好,加样回收率(n=3)分别为98.3%(RSD=0.6%)、103.4%(RSD=1.7%)、97.9%(RSD=0.8%)和97.2%(RSD=1.8%);人参、黄芪、熟地黄、当归、麦冬和肉苁蓉均含有核苷类成分,其中人参、黄芪和当归同时含有尿苷、腺嘌呤、鸟苷和腺苷,并且含量较高。结论:核苷类成分可能是补益中药的一类重要活性成分。
Objective: To analyze nucleosides(uridine,guanosine,and adenosine)and nucleobase(adenine) in 6 tonic traditional Chinese medicines(Radix Ginseng,Radix Astragali,Radix Rehmanniae Preparata,Radix Angelicae Sinensis,Radix Ophiopogonis,and Herba Cistanches) using HPLC-DAD.Methods: The HPLC analysis was performed on a Zorbax SB-Aq(250 mm×4.6 mm,5 μm) column using gradient elution with mobile phase of 0.05% formic acid and acetonitrile.The assay was carried out at a flow rate of 0.5 mL·min-1 at 30 ℃ with the diode-array detection at 260 nm.Results:Uridine,adenine,guanosine,and adenosine showed good linearity in the ranges of 0.30-77.32 μg·mL-1(r=1.000),0.23-58.29 μg·mL-1(r=0.9998),0.35-90.73 μg·mL-1(r=1.000),and 0.61-157.07 μg·mL-1(r=1.000),respectively;The average recoveries(n=3)were 98.3%(RSD=0.6%),103.4%(RSD=1.7%),97.9%(RSD=0.8%),and 97.2%(RSD=1.8%).These compounds could be found in 6 tonic traditional Chinese medicines,but their contents were high in Radix Ginseng,Radix Astragali and Radix Angelicae Sinensis.Conclusions:The nucleosides may be bioactive compounds in tonic traditional Chinese medicines.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2010年第1期33-36,共4页
Chinese Journal of Pharmaceutical Analysis
基金
江苏省方剂研究重点实验室开放课题基金项目(022021014003)
澳门大学基金项目(RG083/07-08S)
关键词
高效液相色谱法
补益中药
尿苷
腺嘌呤
鸟苷
腺苷
HPLC - DAD
traditional Chinese medicines
uridine
adenine
guanosine
adenosine