商陆药材中商陆皂苷甲的含量测定

Content determination of esculentoside A in herbal medicines Radix Phytolaccae

目的:为控制商陆药材的质量,建立HPLC-ELSD法测定商陆药材中商陆皂苷甲的含量。方法:采用Kromasil C18色谱柱(250 mm×4.6 mm,5μm),以甲醇-0.4%醋酸水溶液(70∶30)为流动相,流速1.0 mL.min-1;用蒸发光散射检测器进行检测,ELSD漂移管温度76.5℃,载气流速:2.1 L.min-1。结果:商陆皂苷甲浓度在50.7～507μg.mL-1(r=0.9992)范围内,峰面积自然对数与其浓度自然对数呈良好的线性关系;平均回收率(n=9)为98.7%。结论:该方法简便、准确,专属性强,为2010年版中国药典一部增订商陆含量测定项做了研究。

Objective：To develop an HPLC-ELSD method to determine the content of esculentoside A in herbal medicines Radix Phytolaccae,for controlling the quality.Methods：Kromasil C18（250 mm×4.6 mm,5 μm） column was adopted,with mixture of methanol and 0.4% acetic acid（70∶30） as mobile phase,the flow rate was 1.0 mL·min-1.An evaporative light scattering detector was used.The temperature of drift tube was set at 76.5 ℃,and the gas flow rate was 2.1 L·min-1.Results：The linear range of esculentoside A was 50.7-507 μg·mL-1,the correlation coneffcient was 0.9992;The average recovery（n=9） was 98.7%.Conclusion：This method is simple,accurate,specific.It can offer references for the quality control of herbal medicines Radix Phytolaccae.