三叶草根间菌丝桥传递衰亡根系中磷的作用

UNDERGROUND P TRANSFER AMONG ROOTS OF RED CLOVER VIA VAM HYPHAE LINKS

应用五室方法研究了三叶草根间菌丝桥传递衰亡根系中磷的作用。三叶草生长至１０周切除供体地上部让根系衰亡，１１周时收获样品进行分析测定。结果表明：菌丝桥可以在植株间传递３２Ｐ，从而使受体三叶草地上部磷营养状况得到改善；供体植株地上部切除后有利于３２Ｐ通过菌丝桥从衰亡根系向受体植株的转移，表现为受体植株含磷量有所增加，但对植株的生长影响不大。

Five compartments were designed to examine 32 P transfer via vesicular arbuscular mycorrhizal hyphae link among roots of red clover:the central compartment for the growth of donor and receiver plants and two outer ones for water and hyphae growth.Compartmentation was accomphlished by a 30μm nylon net mesh where hyphae could penetrate and grew while plant roots couldn't . 32 P was mixed into the outer compartment for donor plant and Glomus mosseae was inoculated in the root compartment for donor plant at the beginning of the experiment.There were four treatments in this experiment:mycorrhiza and non mycorrhiza in donor root compartment,detached and intact donor shoot treatments,and each with four replicates.Shoots were detached 10 weeks after planting and plants were harvested one week later.The results demonstrated that hyphae links were formed between roots of donor receiver red clover and could transfer 32 P from donor plant to receiver plant only when roots of donor red clover were inoculated with mycorrhiza fungi.Plant growth and P nutrient status of receiver red clover also got better under this condition. 32 P radioactivity and P concentration of receiver red clover was improved due to the detachment of the donor shoot while plant growth was not affected under mycorrhiza treatment.