人参二酵组皂甙对缺血-再灌注脑组织超微结构及NOS的影响

EFFECTS OF PANAXADIOL SAPONINS ONULTRASTRUCTURES AND NOS OF RAT BRAIN IN CEREBRAL ISCHEMIAREPERFUSION MODEL

目的：研究人参二醇组皂甙（ＰＤＳ）对大鼠脑缺血－再灌注海马超微结构、皮层和海马一氧化氮合酶（ＮＯＳ）活性的影响。方法：双侧颈总动脉阻断和再灌注建立脑缺血－再灌流模型，电镜技术和ＮＡＤＰＨ－ｄ组织化学技术。结果：电镜观察可见，缺血３０ｍｉｎ再灌注２ｈ大鼠海马超微结构发生缺血性病理改变，ＰＤＳ对缺血脑组织病理变化有显著保护作用。ＮＡＤＰＨ－ｄ组织化学实验表明，脑缺血１５ｍｉｎ和再灌注２４ｈ后，皮层及海马ＮＯＳ阳性细胞数目显著增多，ＰＤＳ可显著抑制此增多。结论：ＰＤＳ可通过降低脑内ＮＯＳ的活性，减少脑缺血－再灌注过程中ＮＯ的产生。

AbstractAim: To study the effects of panaxadiol saponins (PDS) on ultrastructures in hippocampus and NOS in hippocampus and cortex of Wistar rats in ischemiareperfusion model. Methods: The cerebral ischemic molde was reproduced by bilateral common carotid arteries occlusion 30 or 15 min and reperfusion 2 or 24 h. Ultrastructures were observed by electromicroscope and NOS was detected by NADPHdiaphorase histochemistry technique. Results: PDS inhibited the morphological changes of hippocampus induced by ischemiareperfusion. The numbers of NOSpositive cells in cortex and hippocampus increased significantly in ischemiareperfusion rats, and PDS inhibited them. Conclusion: PDS protected ultrastructures morphological changes of cerebral from brain ischemiareperfusion injury by inhibited the increase of NOSpositive cells and decreased the NO content during cerebral ischemiareperfusion. KEY WORDS ischemiareperfusion; ultrastructures; NOS; ginseng; saponins; rat