低温保存大鼠肝细胞外向钾电流的变化

CHANGE OF OUTWARD POTASSIUM CURRENT IN RATISOLATED HEPATOCYTES DURING COLD STROAGE

应用全细胞膜片钳制技术观察了低温保存的大鼠肝细胞外向钾电流（Ｉｋ）的变化。结果表明，低温保存大鼠肝细胞的外向钾电流为５．０４±０．８２ｎＡ（ｎ＝１８），大于对照组肝细胞膜电流２．８５±１．２１ｎＡ（ｎ＝３２）（Ｐ＜０．０１）。

y using wholecell patch clamp method, the outward potassium current (Ik) was investigated in isolated rat hepatocytes in control and during cold storage. The current magnitude of Ik during cold storage was 5. 04±0.82 nA (n=18), which was higher than the current magnitude of 2. 85±1. 21 nA in control (P<0. 01). This result showed that the outward potassium curren was improved distinctly in isolated rat hepatocytes during cold storage.