摘要
目的建立测定烂积丸中大黄素、大黄酚含量的HPLC法。方法采用HPLC法。以SHIMADZUVP-ODS为色谱柱;流动相:甲醇-0.05%磷酸(80∶20);检测波长:254nm;流速:1.0mL.min^-1。结果大黄素在4.304-21.52μg范围内线性关系良好,r=0.9996;大黄酚进样量在11.79-58.84 g范围内线性关系良好,r=0.9996。大黄素的加样回收率平均为102.75%,RSD为2.23%(n=6);大黄酚的加样回收率平均为97.67%,RSD为1.66%(n=6)。结论该方法准确、灵敏度高,重现性好。
Objective To establish a HPLC method for the determination of emodin and chrysopanol in Lanji Pills.Methods Octadecylsilane bonded was used as the stationary phase,methanol-0.05% phosphate acid solution(80∶20) as the mobile phase.The column was SHIMADZU VP-ODS with detection wavelength at 254nm and the flow rate was 1.0mL· min^-1.Results The calibration curve of emodin was in good linearity over the range of 4.304~21.52μg(r=0.9996).The calibration curve of chrysopanol was in good linearity over the range of 11.79~58.84μg(r=0.9996).The mean recoveries of emodin and chrysopanol in Lanji Pills were 102.75%(RSD=2.23%) and 97.67%(RSD=1.66 %).Conclusion The method is reliable,accurate and reproducible.
出处
《中国药事》
CAS
2010年第1期78-80,共3页
Chinese Pharmaceutical Affairs