^(19)FNMR在生物医学研究中的应用

19 FNMR in Biomedical Research

核磁共振（ＮＭＲ）是一种无创伤的物理测试方法，它可以直接用于体内和体外的生物样品测定，提供分子水平的信息。正常体内含氟成分很少，测定时无本底信号干扰，因此在体内研究中引进氟代指示剂进行１９ＦＮＭＲ研究是目前普遍采用的方法。１９ＦＮＭＲ可以用来测定药物在体内代谢过程、胞内游离的离子如Ｃａ２＋和Ｍｇ２＋、胞内ｐＨ、氧浓度或氧压力（ｐＯ２）、膜电位、组织温度。

Nuclear Magnetic Resonance(NMR) is a noninvasive method which can be directly used to study biological samples in vivo and in vitro.The information that can be obtained is on the molecular level.The high intrinsic sensitivity of 19 F for detection and lack of significant 19 F background make 19 FNMR studies an attractive strategy by introducing fluorinated indicators for in vivo studies. 19 FNMR has been used to study many physiological and biochemical indexes including metabolism of drugs containing fluorine, intracellular free ions such as Ca 2+ and Mg 2+ , intracellular pH,oxygen concentration or oxygen tension(pO 2),membrane potential,tissue temperature,blood volume and cell volume and so on.