孕鼠维生素D缺乏对子鼠骨发育相关基因BMP-2、Cbfal、Dmp1表达的影响

Effects of vitamin D deficiency during pregnancy on expression of bone development-related genes of offspring

目的:研究大鼠孕期维生素D缺乏对其子鼠骨发育相关基因BMP-2、Cbfa1、Dmp1表达的影响。方法:成熟雌性SD大鼠36只随机分为模型组和对照组各18只,模型组以不含维生素D的大鼠饲料避光喂养,对照组以标准饲料正常光照喂养。两周后与成熟SD雄性大鼠交配,分别取孕20天(E20d)、生后1天(B1d)和生后7天(B7d)子鼠左侧股骨。通过Real-TimePCR方法分别比较模型组和对照组胚胎和新生鼠长骨中BMP-2、Cbfa1、Dmp1基因在3个时间点表达的差异。结果:BMP-2mRNA、Cbfa1mRNA及Dmp1mRNA在E20d、B1d和B7d的表达,模型组均明显低于对照组,差异有统计学意义(P<0.05)。结论:BMP-2、Cbfa1、Dmp1均为骨发育相关基因,孕鼠维生素D缺乏可下调子鼠长骨中BMP-2、Cbfa1、Dmp1的表达水平,从而可能影响子鼠骨骼的发育。

Objective：To study the expression of BMP-2,Cbfa1 and Dmp1 on the femurs of offspring,whose mother is deficient of vitamin D during pregwanoy.Methods：36 adult female SD rats were chosen and allocated randomly into control group（18 rats） and model group（18 rats）.The control group received vitamin D fodder with normal solarization and the model group received non-vitamin D fodder without solarization for 2 weeks.Then the female rats in two groups copulated with normal male rats.The left femurs were collected from the 20-day embryos（E20 d）,the 1-day（B1 d） and 7-day（B7 d） newborns respectively.The expressions of bone developmentrelated genes BMP-2,Cbfa1 and Dmp1 were measured by Real-Time PCR.Results：The expressions of BMP-2,Cbfa1 and Dmp1 mRNA in the model group were lower than those of the control group on E20d,B1d and B7d,respective（P 〈 0.05）.Conclusion：The changes of expressions of bone development-related genes BMP-2,Cbfa1 and Dmp1 on the femurs of offspring are due to vitamin D deficiency of mother during the pregnancy,which would affect the offspring＇s bone development.