压力性尿失禁动物模型两种建立方法比较

Comparsion of two methods to build an animal model of stress urinary incontinence

目的:模仿产伤和破坏大鼠尿道周围组织两种方法建立压力性尿失禁动物模型,并对两种动物模型进行鉴定和比较。方法:雌性成年未生产过的Sprague-Dawley大鼠45只,取5只直接开腹测量尿动力学作为对照组,其余分为A组20只,B组20只。A组行阴道损伤和双侧卵巢切除术,B组行尿道分离术和双侧卵巢切除术。A和B组大鼠各分为4小组,分别称A1-A4和B1-B4,每小组为5只,分别在术后1周,1、2、3月后测量尿动力学指标LPP和ALPP。每组各取2只大鼠行组织学鉴定。结果:与对照值相比A1～A4组LPP值有统计学意义(A1～A3P<0.01,A4P<0.05),ALPP值有统计学意义(A1～A3P<0.01,A4P<0.05),A1～A4组间比较P>0.05,无明显统计学差异。与对照值相比B1～B4组LPP值有统计学意义(B1～B4P<0.05),ALPP值有统计学意义(B1～B4P<0.01),B1～B4组间比较P>0.05,无明显统计学差异。A组和B组组间比较无明显差异(P>0.05)。动物模型尿道组织学上表现为即肌肉成分的萎缩,断裂及神经分布的减少。结论:两种方法均能成功建立SUI动物模型,两种建立方法无明显差异。两种方法建立的模型在3个月内可维持比较良好的稳定性。模拟产伤法具有操作相对简单、风险小、学习曲线短等特点,值得推广。

Objective：To compare the stability and achievement ratio between two rat models of stress urinary incontinence（SUI）, which are developed by simulated birth trauma and urolysis.Methods：Forty-five female virgin Sprague-Dawley rats were divided into 3 groups：five rats were selected randomly for the control group.and remaining rats were divided into group A and B randomly and equally.Rats of group A received vaginal injury operation and bilateral oophorectomy,while urolysis and bilateral oophorectomy was performed on rats of group B.After then,group A and B were divided into four subgroups,each subgroup contained 5 rats.Urodynamic parameters,LPP and ALPP,and histological examinations were carried out post-operation on Week 1,Month 1,Month 2 and Month 3.Results：Compared with reference value,both LPP and ALPP of group A and B decreased significantly（LPP：A1～A3 P 〈 0.01,A4 P 〈 0.05,B1～B4 P 〈 0.05;ALPP：A1～A3 P 〈 0.01,A4 P 〈 0.05,B1～B4 P 〈 0.01）.There was no statistical difference between group A and B.In addition,there was no statistical difference among subgroups from A1 to A4（P 〉 0.05） in group A,and subgroups from B1 to B4（P 〉 0.05） in group B.Histological examinations showed atrophy and disruption of smooth muscle layers,as well as decreased innervation.Conclusion：Both of simulated birth trauma and urolysis can be successfully applied in developing rat model with satisfactory stability of 3 months after operation.Birth trauma model can be established with higher success rates,and has the advantage of low learning curve.Therefore,it can be widely used as the model of SUI.