儿童肺炎支原体肺炎MP-DNA检测和评价

Evaluation of MP-DNA quantitative detection in children with Mycoplasma Pneumoniae Pneumonia

目的:探讨肺炎支原体DNA(MP-DNA),超敏C反应蛋白(SCRP)对诊断支原体肺炎的敏感性和特异性,为临床提供准确快速的诊断依据。方法:采用MP荧光定量PCR法检测患儿呼吸道分泌物标本中的MP-DNA水平,同时检测血清SCRP和MP-IgM的浓度,并与WBC计数进行比较。结果:MP-DNA和SCRP对诊断支原体肺炎的敏感性均高于WBC计数,3个指标的敏感性以SCRP最高;特异性和阳性预测值以MP-DNA最高;阴性预测值和约登指数,MP-DNA和SCRP两者相差无几。结论:MP-DNA检测对肺炎支原体的早期诊断有一定价值。

Objective：To explore diagnostic evaluation of sensitivity and specificity of MP-DNA and SCRP on children with MP pneumonia,and provide accurate and quick diagnostic proof to clinic.Methods：Children＇s respiratory secretion samples were collected for fluorescent quantitative PCR for MP-DNA,and the serum＇s concentrations of SCRP and MP-IgM were detected by immunobiochemistry methods,and the three indexes were compared with WBC＇s count.Results：MP-DNA and sCRP were more sensitive in diagnosis of MPP compared with WBC count.Among the three indexes,the sCRP was the most sensitive,and MP-DNA had highest specificity and positive predictive value,and there were no differences among the indexes on negative predictive value and Youden＇s index.Conclusion：MP-DNA is evaluable in early and accurate diagnosis on MP infection.