江苏省113株大肠杆菌O157:H7的扩增片段长度多态性分析

Amplified fragment length polymorphism of 113 enterohemorrhagic Escherichia coli O157:H7 isolated in Jiangsu Province

目的:分析江苏省不同地区和年代分离的EscherichiacoliO157:H7(E.coliO157:H7)之间的同源性。方法:用扩增片段长度多态性(amplified fragment length polymorphism,AFLP)分析法对113株E.coliO157:H7菌株进行分子分型,采用软件BioNumerics Version4.0对分型数据进行处理和分析。结果:113株E.coliO157:H7共分37个型别,不同来源的菌株存在相同的基因型别,不同的地区和物种之间存在着相互传播。结论:AFLP可以用于对不同来源的E.coliO157:H7进行分子分型。

Objective：To identify the homology among enterohemorrhagic Escherichia coli（EHEC） isolated from different years, cites and hosts.Methods：Amplified fragment length polymorphism（AFLP） was used for the molecular subtyping of 113 E.coli O157：H7 strains.The AFLP patterns were analyzed by BioNumerics version 4.0 software.Results：113 isolates showed 37 patterns.Strains isolated from different years and hosts may have the same patterns.Conclusion：From the above observations,it is evident that AFLP is useful in the molecular typing of E.coli O157：H7 isolated from different sources.