差异显示技术及其进展

Differential Display and Its Process

差异显示技术是在转录水平上研究基因表达差异的有效方法。它通过一系列的锚定引物与随机引物组合把不同种细胞的ｍＲＮＡ进行分组反转录及ＰＣＲ扩增，在相邻泳道上显示扩增结果，通过比较电泳图谱找出差异。差异显示技术建立至今仅五年时间便在分析基因表达差异、绘制遗传图谱、分离特异性表达基因、临床诊断遗传疾病等方面被广泛应用，并不断得到改进和完善。

Differential display is a useful technique for analyzing differential gene expression on transcription level.Differentially expressed mRNAs are identified by performing reverse transcription with anchored primer and subsequent PCR with sets of anchored primer and arbitrary primer and next comparing in a side by side fashion after electrophoring in adjacent lanes of a sequencing gel.This technique is widely applied not only in many basic research fields,for instance,analyzing the differential gene expression drawing a gene map or isolating the species or tissue specific gene,but also in clinic diagnosis.It has been developed and optimized since established.