阳荷蛋白质提取工艺优化及锌赋存状态研究

Extraction of Protein and Speciation Analysis of Zinc in Zingiber striolatum

在单因素试验基础上,采用正交试验对阳荷蛋白质的提取工艺条件进行筛选,得出最优提取条件:在60℃条件下,用40倍体积于阳荷粉的pH9.0的磷酸缓冲液恒温提取60min。同时对阳荷中锌赋存状态进行研究,结果表明,在供试阳荷中,锌以有机态为主,占总锌含量80%以上,蛋白锌占有机锌65%以上。

The optimal extraction process of protein from Zingiber striolatum determined using single factor method coupled with orthogonal array design was that Zingiber striolatum powder was extracted with PBS （pH 9.0） at 60 ℃ for 60 minutes with a solid-to-solvent ratio of 1：40 （g/mL）. Additionally, speciation analysis of zinc in Zingiber striolatum was carried out. Results revealed that organic zinc constituted a major fraction in Zingiber striolatum, which accounted for above 80% of total zinc and comprised above 65% protein-binding zinc.