火绒草提取物抗氧化活性的研究

Antioxidant Activity of Extracts from Leontopodium leontopodioides

测定火绒草水提物和醇提物中多酚及总黄酮含量,证实水提物和醇提物中含有以黄酮类为主要成分的多酚类物质。通过测定火绒草提取物的总还原力,超氧阴离子自由基(O2.)、羟自由基(.OH)清除能力、脂质过氧化抑制作用、清除亚硝酸盐自由基和亚硝胺阻断率,表明火绒草水提物和醇提物具有较强的还原性和清除.OH和O2.的活性,且醇提物的作用比水提物更有效;对脂质过氧化的抑制率达到60%以上。火绒草提取物具有较强的清除亚硝酸钠和阻断亚硝胺合成的能力,水提物对亚硝酸盐最大清除率为77.7%,醇提物对亚硝胺合成的阻断率98.0%。

In this study,water and ethanol extracts from Leontopodium leontopodioides were confirmed to be polyphenols,which was mainly composed of flavonoids.The antioxidant activities of the water and ethanol extracts were evaluated by in vitro total reducing power,superoxide anion,hydroxyl and sodium nitrite free radical scavenging capacity,lipid peroxidation inhibition,and nitrosamine synthesis blocking assays.Both the extracts exhibited strong reducing power and scavenging effect against free radicals.Above 60% inhibition rate against lipid peroxidation was observed in the ethanol extract.Moreover,both the extracts revealed strong capacity to scavenge sodium nitrite and block nitrosamine synthesis.The maximum scavenging rate of the water extract against sodium nitrite was 77.7% and the blocking rate of the ethanol extract against nitrosamine synthesis 98%.