利用酒糟浸出液制备细菌纤维素

Fermentation of Lixivium of Distiller’s Grains for Bacterial Cellulose Preparation

选用3种酒糟浸出液作为主要原料,利用木醋杆菌发酵制备细菌纤维素。结果表明:酒糟浸出液营养丰富,加入稻壳的白酒丢糟浸出液更适合发酵,在其中添加2%蔗糖、pH5.0和30℃恒温静止发酵15d后细菌纤维素的产量为2.7g/L,发酵后的pH值有变大的趋势。利用扫描电镜、傅里叶变换红外、X射线衍射对细菌纤维素的结构进行表征,证明产物是细菌纤维素,纤维直径在20～100nm之间。

Bacterial cellulose（BC） was prepared from lixivium of three distiller’s grains fermented by Acetobacter xylinum.Results showed that the lixivium from three distiller’s grains was rich in nutrients.However,the lixivium from distiller’s grains with rice husk was more suitable to produce BC.The yield of BC was 2.7 g/L when the lixivium with the addition of 2% sucrose was conducted to static fermentation at pH 5.0 and 30 ℃ for 15 days.The pH exhibited an increase trend after fermentation.The structural characteristics of BC were demonstrated using scanning electron microscope（SEM）,Fourier transform infrared（FT-IR） spectrum and X-ray diffraction（XRD） with the diameter between 20 nm and 100 nm.