山楂黄酮提取物的抗氧化活性和对癌细胞生长抑制作用

Hawthorn Flavonoid Extract:Antioxidant Activity and Growth Inhibition Effect on Cancer Cells

目的:研究山楂(Crataegus pinnatifida Bunge)黄酮提取物的抗氧化能力以及对人肝癌Hep-G2细胞和人肠癌Caco-2细胞生长抑制作用。方法:比较测定总黄酮含量的直接法和两种铝盐显色法,进一步采用硝酸铝盐显色法测定提取物总黄酮含量,ABTS法和DPPH法分析提取物的抗氧化活性,MTT法评价山楂黄酮提取物对细胞生长的抑制效果。结果:山楂黄酮提取物中黄酮含量占干质量的98%,清除ABTS和DPPH自由基的能力随提取物剂量的增加而升高,EC50分别为88μg/mL和112μg/mL,而标准品Trolox的EC50清除ABTS和DPPH自由基的能力分别为111μg/mL和118μg/mL。山楂黄酮提取物能够有效地抑制癌细胞的生长,且对Hep-G2细胞的抑制效果更强,Hep-G2细胞和Caco-2细胞的IC50分别约为115μg/mL和376μg/mL。结论:山楂黄酮提取物黄酮含量高,抗氧化能力强,有抑制癌细胞的作用,是一种有效的天然抗氧化剂。

Objective： To study antioxidant activity of hawthorn（Crataegus pinnatifida Bunge） flavonoid extract（HFE） as well as its growth inhibition effect on Hep-G2 and Caco-2 cells.Methods： Total flavonoid content was determined by NaNO2-Al（NO3） 3 colorimetric method.Antioxidant activity was evaluated by 2,2’-azinobis（3-ethylbenzothiazoline-6-suslfonic acid（ABTS） and 1,1-diphenyl-2-picryl-hydrazyl（DPPH） free radical scavenging assays.Cell growth inhibition effect of HFE was assessed by MTT assay.Results： Flavonoids were dominant in HFE with a content of 98%（calculated on the basis of dry weight）.Both ABTS and DPPH scavenging capacities exhibited an increase with increasing HFE concentration.The EC50s of HFE was 88μg/mL and 112μg/mL in ABTS and DPPH assays,respectively;while those of Trolox were 111μg/mL and 118μg/mL.Cell growth of Hep-G2 and Caco-2 cells were remarkably inhibited by HFE in a dose-dependent manner.Whereas,HFE had stronger growth inhibition effect against Hep-G2 cells than against Caco-2 cells.The IC50s of HFE were 115 μg/mL and 376μg/mL for Hep-G2 and Caco-2 cells,respectively.Conclusion： HFE has high content of flavonoids,strong antioxidant activity,and strong inhibitory effect against cancer cell growth.Therefore,it has a promising potential as an effective natural antioxidant and an anticancer functional component.