摘要
目的:研究山楂(Crataegus pinnatifida Bunge)黄酮提取物的抗氧化能力以及对人肝癌Hep-G2细胞和人肠癌Caco-2细胞生长抑制作用。方法:比较测定总黄酮含量的直接法和两种铝盐显色法,进一步采用硝酸铝盐显色法测定提取物总黄酮含量,ABTS法和DPPH法分析提取物的抗氧化活性,MTT法评价山楂黄酮提取物对细胞生长的抑制效果。结果:山楂黄酮提取物中黄酮含量占干质量的98%,清除ABTS和DPPH自由基的能力随提取物剂量的增加而升高,EC50分别为88μg/mL和112μg/mL,而标准品Trolox的EC50清除ABTS和DPPH自由基的能力分别为111μg/mL和118μg/mL。山楂黄酮提取物能够有效地抑制癌细胞的生长,且对Hep-G2细胞的抑制效果更强,Hep-G2细胞和Caco-2细胞的IC50分别约为115μg/mL和376μg/mL。结论:山楂黄酮提取物黄酮含量高,抗氧化能力强,有抑制癌细胞的作用,是一种有效的天然抗氧化剂。
Objective: To study antioxidant activity of hawthorn(Crataegus pinnatifida Bunge) flavonoid extract(HFE) as well as its growth inhibition effect on Hep-G2 and Caco-2 cells.Methods: Total flavonoid content was determined by NaNO2-Al(NO3) 3 colorimetric method.Antioxidant activity was evaluated by 2,2’-azinobis(3-ethylbenzothiazoline-6-suslfonic acid(ABTS) and 1,1-diphenyl-2-picryl-hydrazyl(DPPH) free radical scavenging assays.Cell growth inhibition effect of HFE was assessed by MTT assay.Results: Flavonoids were dominant in HFE with a content of 98%(calculated on the basis of dry weight).Both ABTS and DPPH scavenging capacities exhibited an increase with increasing HFE concentration.The EC50s of HFE was 88μg/mL and 112μg/mL in ABTS and DPPH assays,respectively;while those of Trolox were 111μg/mL and 118μg/mL.Cell growth of Hep-G2 and Caco-2 cells were remarkably inhibited by HFE in a dose-dependent manner.Whereas,HFE had stronger growth inhibition effect against Hep-G2 cells than against Caco-2 cells.The IC50s of HFE were 115 μg/mL and 376μg/mL for Hep-G2 and Caco-2 cells,respectively.Conclusion: HFE has high content of flavonoids,strong antioxidant activity,and strong inhibitory effect against cancer cell growth.Therefore,it has a promising potential as an effective natural antioxidant and an anticancer functional component.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第3期220-223,共4页
Food Science
基金
新加坡国立大学与中国农业大学国际合作交流基金资助项目
"十一五"国家科技攻关项目(2006BAD27B04)