蓝靛果花色苷对高脂膳食诱导肥胖大鼠脂代谢和抗氧化能力的影响

Effect of Lonicera caerulea Anthocyanin on Lipid Metabolism and Antioxidant Function in High Fat Diet-induced Obese Rats

目的:研究蓝靛果果渣花色苷(Lonicera caerulea residue anthocyanin,LCRA)对高脂膳食诱导肥胖大鼠脂类代谢和抗氧化能力的影响。方法:将大鼠随机分为基础对照,肥胖模型对照和低、中、高剂量花色苷共5组,即4.0、40.0、120mg/kg bw.d的花色苷灌胃,基础对照组和肥胖模型对照组分别灌胃生理盐水(1.2g/kg bw.d);连续28d后,分别测定大鼠体质量、脂肪、肝脏质量,血清总胆固醇(TC)、总甘油三酯(TG)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C)含量以及肝脏脂肪酶(LPS)、肝脂酶(HL)、脂蛋白脂肪酶(LPL)、超氧化歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)的活性和脂质过氧化物(MDA)含量。结果:与肥胖模型对照组相比,LCRA能显著降低肥胖模型大鼠血脂水平,使肝脏LPS、HL、LPL、SOD和GSH-Px活性明显增强,MDA的生成量显著减少。结论:LCRA对高脂膳食诱导肥胖大鼠脂代谢有明显调节作用,可有效减少肝脏过氧化损伤,预防动脉硬化的发生。

Objective： To study the effect of anthocyanin extracted from Lonicera caerulea residue（LCRA） on lipid metabolism and antioxidant function in high fat diet-induced obese rats.Methods： Rats were divided into 5 groups,inluding normal control group,obesity model group and LCRA low-,medium-and high-dose groups treated with LCRA at doses of 4.0,40.0 mg/kg bw.d and 120 mg/kg bw.d.Normal control and obesity model groups were fed normal saline at the dose of 1.2 g/kg bw.d for 28 consecutive days.After administration,body and liver weight,body fat percentage,total triglycerides（TG）,total cholesterol（TC）,high density lipoprotein cholesterol（HDL-C）,low density lipoprotein cholesterol（LDL-C）,activities of HL,LPL,LPS,SOD and GSH-Px,and MDA content in liver were measured.Results： Compared with obesity model group,LCRA treated groups exhibited a significant reduction in serum lipid level and MDA content in liver and a significant increase in the activities of HL,LPL,LPS,SOD and GSH-Px in liver.Conclusion： LCRA can improve lipid metabolism in high fat diet-induced obese rats,which reveals strong functions for reducing peroxidative liver injury and preventing arteriosclerosis.