黑豆多肽对D-半乳糖衰老小鼠抗氧化能力的影响

Antioxidant Function of Black Soybean Peptide in D-Galactose-induced Senescent Mice

采用蛋白酶复合水解黑豆分离蛋白制备黑大豆多肽(BSP),将50只昆明小鼠随机分为5组(对照组、模型组、VC阳性组和低、高剂量组),除对照组外,其余4组分别腹腔注射400mg/kg bw.d D-半乳糖建立氧化衰老模型;多肽组分别灌胃BSP(400、800mg/kg bw.d),实验周期56d。实验结束后,处死小鼠,取脾、肾、肝脏及胸腺称质量,计算脏器指数;测定血清及肝脏中丙二醛(MDA)含量和谷胱甘肽过氧化物酶(GSH-Px)活性以及肝脏脂褐质(LPF)含量。结果表明,BSP能使小鼠胸腺指数和脾脏指数升高,血清和肝脏MDA含量明显下降,GSH-Px活力显著提高;并且使肝组织中LPF含量明显下降。提示BSP能显著提高亚急性衰老小鼠的抗氧化能力,尤以高剂量组效果最好,具有一定延缓衰老的作用。

In order to investigate the antioxidant function of black soybean peptide（BSP） in D-galactose-induced senescent mice,fifty Kunming mice were divided into five groups,designated as control group,oxidative injury model group,VC positive control group,and BSP high-and low-dose groups.Except the control group,all other groups were intraperitoneally injected with D-galactose solution at the daily dose of 400 mg/kg bw.d for 56 successive days.At the same time,BSP-treated mice were administered BSP by gavage at the daily dose of 400 mg/kg bw.d and 800 mg/kg bw.d,respectively.Malondialdehyde（MDA） content,glutathione peroxidase（GSH-Px） activity and lipofuscin（LPF） content in serum or in liver were assayed.Moreover,the indexes of spleen,kidney,liver and thymus gland were also determined.Results showed that thymus index,spleen index and MDA content exhibited a significant decrease.While GSH-Px activity revealed a significant decrease both in serum and in liver of oxidative injury mouse model.Both 400 mg/kg bw.d and 800 mg/kg bw.d BSP administrations resulted in a significant increase in thymus index,spleen index and GSH-Px activity in serum or liver and a significant decrease in MDA content.Furthermore,BSP at the dose of 800 mg/kg bw.d also resulted in a significant decrease in LPF content in liver.Therefore,BSP has obvious antioxidant activity in vivo due to its free radical scavenging activity and improved immune organ index for accomplishing anti-aging effect in a dose-dependent mode.