冰冻血小板融化复苏后稳定性分析

Study on the stability of melt resuscitative frozen platelets

目的探讨冰冻血小板融化复苏后的稳定性。方法将血小板分别置于-80％和-90℃～-120℃保存，观察冰冻血小板融化复苏后纤维蛋白及絮状聚集物的情况。结果80℃保存血小板纤维蛋白及絮状聚集率为23％，-90℃～-120℃保存血小板纤维蛋白及絮状聚集率为0。血小板保存温度不同，纤维蛋白及絮状聚集发生率的差异有显著性（χ^2=219．64，P〈0．01），冰冻血小板冷冻速度和保存温度对血小板质量的影响有显著性。结论冰冻血小板于-90℃～120℃保存的稳定性比较好。

Objective To study the stability of melt resuscitative frozen platelets. Methods Frozen platelets were stored in different temperature, at-80℃ and-90℃ --120℃ respectively. The formation of fibrin and irreversible aggregation in these melt resuscitative frozen platelets were observed. Results The rate of fibrin and irreversible aggregation was 23% when platelets were stored at-80℃. The rate of fibrin and irreversible aggregation was 0 when platelets were stored at-90℃ - -120℃. In different temperature the formation of fibrin and irreversible aggregation were significantly different（χ^2 = 219. 64, P 〈 0. 01 ）. The maintain temperature and frozen velocity inplatelets could affect the quality of platelets. Conclusion Frozen platelets stored at-90℃ -- 120℃ have good stability ofmelt resuscitative frozen platelets by preservation the labile coagulation factors in platelets.