福氏志贺菌4c型病原学特性及毒力基因检测

Pathogenic characteristics and virulence genes of Shigella flexneri 4c in in Minhang district of Shanghai

目的：了解上海市闵行区福氏志贺菌4 c型生化特征、对抗生素的耐药性及毒力基因携带情况,为痢疾的防治工作提供依据。方法：对本地区2007年-2008年各肠道门诊监测点腹泻病人中分离到的福氏志贺菌4 c型菌株,用ATB进行生化检测,K-B法进行耐药性检测,PCR扩增进行setlA、set1B、sen、ial和ipaH5种毒力基因检测。结果：36株福氏志贺菌4 c型100%发酵葡萄糖、91.7%发酵甘露醇,27.8%菌株吲哚阳性。对萘啶酸耐药率高达100.0%,利福平、复方新诺明、四环素、氨卞西林的耐药率为97.2%、88.9%、83.3%、83.3%,80.6%菌株耐受一半及以上抗生素。setlA、set1B、sen、ial、ipaH基因携带率均为91.7%、91.7%、100.0%、100.0%、100.0%。结论：福氏4 c志贺菌多重耐药情况较为严重,敏感药物有庆大霉素和第三代头胞类药物。菌株毒力基因携带率高,具有很强的致病性。

Objective：To investigate the biochemical characteristics,antibioticresistance and virulence genes of Shigella flexneri 4c isolates in in Minhang district of Shanghai.Methods：Biochemical identification of the strains was performed using API 32E,serotyping using slide agglutination,antimicrobial susceptibility testing using the Kirby-Bauer method and detection of setlA,setlB,sen,ial,ipaH virulence genes using PCR.Results：The S.flexneri 4c isolates,fermenting glucose and with 91.7% strains fermenting mannitol,were found 100% resistant to nalidixicacid,97.2% to rifampicin,88.9% to SMZ/TM,83.3% to tetracycline,83.3% to ampicillin,80.6% isolates possessed resistance to more than a half of antibiotic species.Presence of setlA,setlB,sen,ial ipaH virulence genes was identified among 91.7%,91.7%,100.0%,100.0% and 100.0% of tested isolates.Conclusion：Strong multidrug resistance of S.flexneri 4c isolates has been found in the present study.Susceptibility to gentamicin and norfloxacin was identified.Presence of virulence genes associated with strong pathogenicity is found in a high proportion of isolates.