紫外可见分光光度法检测血液中碳氧血红蛋白饱和度及其变化

HbCO in blood of carbon monoxide poisone bodies detected by UV/Visible spectrophotometry and concentration changes of HbCO%

目的:探索室温条件下一氧化碳中毒血液中HbCO饱和度变化规律。方法:取一氧化碳中毒血液样本,用0.1%的Na2CO3稀释200倍并加入Na2S2O4少许,紫外可见分光光度计双波长吸光度比值法(还原法)测定。结果:室温条件下,随保存时间的延长CO中毒死亡者、中毒大鼠及中毒家兔血液中HbCO饱和度在3个月内均呈下降趋势,其下降速率与HbCO饱和度大小无相关关系。结论:CO中毒死亡后的尸体血液中HbCO含量在室温条件下会随保存时间的延长而发生变化。

Objective：To explore the concentration changes of HbCO in blood at room temperature.Methods：Blood samples was diluted with 0.1% Na2CO3,to which sodium hydrosulfite was added.Its absorption at 538nm and 555nm were determinated by visible spectrophotometer.Results：In three months after death,the changing trend of HbCO content was descending in blood.The elimination coefficient of carbon monoxide was ascending and the elimination rate had no correlation with HbCO%.Conclusion：The carboxyhemoglobin contents in blood of carbon monoxide poisone bodies was changed at room temperature.