血清抗Ⅱ型胶原抗体与自身免疫病疾病活动性关系的探讨

Relationship between level of serum anti-type Ⅱ collagen antibody and autoimmune disease

目的:通过观察人血清CCP与抗Ⅱ型胶原抗体之间的关系,比较不同程度RA患者抗Ⅱ型胶原抗体的诊断界值,研究抗Ⅱ型胶原抗体应用于RA的临床价值。方法:采用ELISA法检测血清抗NatⅡIgG、抗CCP,用速率散射比浊法检测类风湿因子(RF)、C-反应蛋白(CRP),放射免疫法检测系统性红斑狼疮(SLE)患者血清中的抗双链DNA(dsD-NA)。结果:RA患者血清中抗NatⅡIgG水平与SLE,骨关节坏死,正常对照组之间差别有统计学意义(P<0.05)。RA患者血清抗NatⅡIgG水平与CCP、CRP含量呈正相关(相关系数r分别为0.007和0.023)。结论:抗NatⅡ抗体水平可作为RA及SLE患者关节损伤的辅助诊断指标。

Objective：By observing the relationship between the anti cyclic citrullinated peptides and the anti-type Ⅱ collagen antibody in human serum,to compare the cut-off value of anti-type Ⅱ collagen antibody in different state of rheumatic arthritis,in order to study the applied value about detection of serum anti-type Ⅱ collagen antibody in rheumatic arthritis.Methods：All serum samples were detected the anti-type Ⅱ collagen antibody and the anti cyclic citrullinated peptides level by means of ELISA method,the rheumatoid factors and C-reactive protein level by means of rate nephelometry method,the anti-double stranded deoxyribonucleic acid antibodies by means radioimmuno assay.Results：There was significant difference amoung the groups with rheumatoid arthritis,systemic lupus erythematosus,knee osteoarthritis and the control group about the level of serum anti-type Ⅱ collagen antibody.The anti-type Ⅱ collagen antibody in serum of rheumatoid arthritis patients were correlated directly with the level of the anti cyclic citrullinated peptides and C-reactive protein.Conclusion：It was assistanted for diagnostic of rheumatoid arthritis and systemic lupus erythematosus patients whose joint was damaged to detected the anti-type Ⅱ collagen antibody.