耐力训练对AMPKα2基因敲除小鼠骨骼肌CPT-1表达的影响

Effect of Endurance Training on Skeletal Muscle CPT-1 Expression of α2-AMPK Whole-body Knockout Mice

目的:研究耐力训练对AMPKα2基因敲除小鼠骨骼肌CPT-1mRNA和蛋白表达的影响,探讨耐力训练中AMPKα2调节脂肪酸代谢的可能途径。研究方法:两月龄C57BL/6J野生(WT)和AMPKα2基因敲除(KO)小鼠各20只,各自随机分为安静对照组,耐力训练组,每组10只。两训练组进行4周,每天1 h,速度为12 m/min的跑台训练,测定股四头肌CPT-1 mRNA和蛋白表达以及血清FFA、TG水平。结果:4周耐力训练之后,WT鼠和KO鼠骨骼肌CPT-1 mRNA和蛋白表达,与安静对照组相比均显著升高,且两训练组之间无显著差异。结论:耐力训练中AMPKα2不是CPT-1的唯一上游调节因子,可能有其他途径参与CPT-1表达的调节。

Objective： To study the effect of endurance training on skeletal muscle CPT-1 mRNA and protein expression of α2-AMPK whole-body knockout （KO） mice. Meanwhile, we also explore the possible role of AMPKα2 in regulating fatty acid oxidation in endurance training. Methods： C57BL/6J wild-type （WT） mice （n=20） and α2-AMPK whole-body knockout （KO） mice （n=20）, two months old, were randomly subdivided into control groups and exercise groups. Quadriceps femoris muscle was removed after 4 weeks tradmill running （12m/min, 1h/day）. Expression of CPT-1 mRNA and protein, blood serum FFA and TG levels were measured. Results： After 4 weeks endurance training, skeletal muscle CPT-1 mRNA and protein expression of WT and KO mice were significantly higher than control groups. At the same time, there were no significant differences between two exercise groups. Conclusion： AMPKα2 is not the only upstream regulatory factor of CPT-1 in endurance training, and there could have other factors which regulate CPT-1 mRNA and protein expression during this kind of training.