E2F-1蛋白在肺鳞状细胞癌及腺癌中的表达及临床病理学意义

Clinicopathological significance and expression of E2F-1 protein in squamous cell carcinoma and adenocarcinoma of lung

目的:研究E2F-1蛋白在肺鳞状细胞癌及腺癌中的表达情况,探讨其在肺癌发生发展中的作用机制及其与临床病理学特征间的关系。方法:应用免疫组化ElivisionTMsuper二步法检测71例肺鳞状细胞癌、腺癌及相应癌旁组织中E2F-1蛋白的表达情况。结果:肺鳞状细胞癌及腺癌组织中E2F-1蛋白的阳性表达率为83.1%(59/71),显著高于癌旁组织中的8.5%(6/71)(P<0.05);E2F-1蛋白表达随癌组织分化程度的减低有升高的趋势,在肺腺癌组织中(91.4%)的表达虽高于鳞状细胞癌中的表达(75.0%),但与组织分化、组织类型差异均无统计学意义,且与患者年龄、性别、肿瘤大小、有无淋巴结转移及TNM分期等差异无统计学意义(P>0.05)。结论:E2F-1的过表达与肺鳞状细胞癌及腺癌的发生发展密切相关。

Objective：To investigate the expressions and clinicopathological characteristics of E2F-1 protein in the squamous carcinoma and adenocarcinoma of lung.Methods： The expressions of E2F-1 protein of 71 cases of squamous cell carcinoma and adenocarcinoma of lung and the related peritumoral lung tissues were detected by using immunohistochemical technique.Results：： The positive rates of E2F-1 protein expression were 83.1%（59/71） in cancerous tissue,which was significantly higher than 8.5%（6/71） in peritumoral lung tissues（P0.05）.Although E2F-1 protein expression rates were higher in adenocarcinoma than squamous cell carcinoma and negative correlation with differentiation degree,they were no significance difference in histological type and differentiation degree.And the expression rates of E2F-1 protein were no correlation with age,gender,tumor size,lymphnode metastasis and TNM stage of patients（P0.05）.Conclusion： Abnormal expression of E2F-1 protein may play an important role in the tumorigenesis and progression for the squamous cell carcinoma and adenocarcinoma of lung.