呼吸道病原体多重实时荧光定量PCR的检测

Multiplex Real-time Fluorescence Quantitative PCR for Detection of Respiratory Tract Infection Pathogen

目的建立多重实时荧光定量PCR检测方法,为实现对呼吸道病原体的快速、灵敏特异性检测。方法采用经报道的引物和探针对合胞病毒、腺病毒等8种呼吸道病原体进行多重实时荧光定量PCR检测。结果多重荧光定量PCR阳性检测率高达57.8%,无假阳性及假阴性,特异性强。结论多重实时荧光定量PCR具有经济、快速、特异性强等优点,在呼吸道病原体检测方面有巨大应用价值。

OBJECTIVE To make the detection of respiratory pathogen rapid, sensitive and specific by use of the multiplex real-time fluorescence quantitative PCR. METHODS We recommended the primers and probes for detection of rospiratory syncytial virus（RSV）, adenovirus （AdV）, fluenza virus（Flu）, parainfluenza virus（PIV）, metapneumo virus（MPV）, rhinovirus （RHV）, Mycoplasma pneumoniae （Mp） and Chlamydia pneumoniae （ Cp） in multiplex real-time fluorescence quantitative PCR assay. RESULTS Among the samples 57.8% were detected out the pathogens and the specificity was 100. 0%. CONCLUSIONS The multiplex real-time fluorescence quantitative PCR assay indicated optimal specificity and sensitivity and deserves to be applied in diagnosis of respiratory tract infection pathogens.