代谢组学法研究三聚氰胺对儿童尿液代谢的影响

Metabonomics Research of the Influence of Melamine to the Urine Metabolism of the Children Based on UPLC/TOF-MS

运用代谢组学方法研究了三聚氰胺对儿童尿液代谢的影响.通过超高效液相色谱-飞行时间质谱(UPLC/TOF-MS)法分析儿童尿样的代谢指纹图谱,质谱数据采用MarkerLynx软件处理,然后使用主成分分析和偏最小二乘判别分析法分析病例组和正常对照组之间的代谢物谱差异,并通过变量重要性投影(VIP)选取潜在的生物标志物,结合质谱同位素分析和数据库检索对潜在的生物标志物进行鉴定.结果表明,三聚氰胺通过肾结石导致的物理性损伤干扰了柠檬酸代谢.代谢组学法能够应用于三聚氰胺导致的代谢异常的研究及三聚氰胺导致肾损伤的无创检测.

The metabonomics method was employed to investigate the possible influence of melamine to the urine metabolism of the children. Urine samples of 7 melamine affected children and 570 healthy children were collected from the Children Hospital of Changchun. The profiles of the collecteed urine samples were obtained with ultra performance liquid chromatography coupled with time-of-flight mass spectrometry（UPLC/TOF-MS）. The data were firstly processed with the software MarkerLynx, then analyzed with principle component analysis（PCA） and partial least squares discriminant analysis （PLS-DA）. The potential biomarkers were screened out according to the VIP（ variable importance in projection） value and identified with i-fit and database. Most of the metabolites of significant intergroup differences were finally found to be in the tricarboxylic acid cycle. According to the biological functions of the obtained potential biomarkers, it was deduced that the citric acid metabolism was disturbed by melamine through its kidney injury effect. The results suggest that the metabonomics method can be utilized to investigation of the influence of melamine to the metabolism as well as the noninvasive examination of the melamine caused kidney injury.