反胶束法提取花生蛋白后萃工艺的优化

Optimization of the backward extraction of protein from peanut by reverse micelles

以琥珀酸二(2-乙基己基)酯磺酸钠(AOT)-异辛烷-氯化钾缓冲溶液为前萃体系,对从前萃体系中提取花生蛋白的后萃工艺条件进行了研究。考察了后萃时间、后萃温度、缓冲液pH、KCl浓度对花生蛋白后萃率的影响,并在单因素试验基础上,通过正交试验确定后萃最佳工艺条件为:后萃时间50 min,后萃温度40℃,缓冲液pH9.0,KCl浓度1.0 mol/L。在此最佳工艺条件下,花生蛋白后萃率达到86.49%。

The backward extraction of peanut protein from forward extraction solution was studied. The reverse micellar systems were formed by sulphosuccinic acid his （2 -ethylhexyl ） ester sodium salt （AOT） ,isooctane and KCl buffer solution. The effects of extraction time ,extraction temperature, pH and KCl concentration on the backward extraction ratio were tested. On the basis of single - factor experi- ments,the optimum technical condition was obtained by orthogonal experiments. The results showed that the highest backward extraction ratio of protein were reached at the condition of extraction time 50 rain, extraction temperature 40 ℃ ,pH 9.0 and KCl concentration 1.0 mol/L. Under this optimum condition, the backward extraction ratio of protein reached 86.49%.