还原型谷胱甘肽对慢性乙型肝炎患者血清肝纤维化指标和细胞因子的影响

Influence of Reduced Glutathione on Serum Liver Fibrosis Index and Cytokines in Patients with Chronic Hepatitis B

目的:探讨还原型谷胱甘肽(GSH)对慢性乙型肝炎(CHB)患者血清肝纤维化指标及细胞因子的影响。方法:96例CHB患者随机分为对照组和治疗组(GSH组),治疗前后采用酶联免疫吸附试验(ELISA)双抗体夹心法和放射免疫分析法(RIA)测定血清转化生长因子-β1(TGF-β1)、肿瘤坏死因子-α(TNF-α)、玻璃酸(HA)、Ⅳ型胶原(C-Ⅳ)、层黏连蛋白(LN)以及丙氨酸氨基转移酶(ALT)、天门氡氨酸氨基转移酶(AST);另设30例健康体检者为健康组。结果:CHB患者血清ALT、AST、HA、C-Ⅳ、LN和TGF-β1、TNF-α水平较健康组显著升高(P<0.01)。采用GSH治疗8周后ALT、AST、HA、C-Ⅳ、LN和TGF-β1、TNF-α水平较治疗前及对照组均有显著降低(P<0.01)。结论:GSH能够降低CHB患者血清TGF-β1、TNF-α水平,具有抗炎及抗肝纤维化作用。

OBJECTIVE： To study the influence of reduced glutathione （GSH） on serum liver fibrosis index and cytokines in patients with chronic hepatitis B （CHB）. METHODS： 96 patients with CHB were randomly divided into the treatment group （treated with GSH） and control group. The serum levels of TGF-β1, TNF-α, hyaline acid（HA）, type Ivcollagen （C-Ⅳ）, laminin （LN）, Alanine aminotransferase（ALT）and Aspartate Aminotransferase （AST） were measured by specific-ELISA and RIA assay before and after treatment. Healthy group made up of 30 healthy volunteers. RESULTS： Before treatment the serum levels of ALT, AST, TGF-β1, TNF-α, HA, C-Ⅳ and LN in CHB group were significantly increased, as compared with heatthy group （P〈0.01）. After 8 weeks of treatment, the serum levels ofALT, AST, TGF-β1, TNF-α, HA, C-Ⅳ and LN in treatment group were significantly reduced, as compared with control group and before treatment （P〈0.01）. CONCLUSION： GSH could lower the serum levels of TGF-β1 and TNF-α in patients with CHB and inhibit liver fibrosis.