一种利用染色体显微切割和微克隆获得小麦基因探针的方法

A Method of Using Chromosome Microdissection to Obtain Gene Probe in Wheat

利用染色体显微切割和微克隆技术对普通小麦钢８２１２２（Ｔｒｉｔｉｃｕｍａｅｓｔｉｖｕｍ２ｎ＝４２）染色体长臂１Ｄ染色体进行切割和ＰＣＲ扩增，得到了高分子量麦谷蛋白（ＨＭＷＧＳ１）１Ｄｘ５亚基基因片段。

The 1D chromosome long arms of common wheat Gang 82 122 were microdisseted with the cell manipulation instrument,and HMW GS1Dx5 gene 5′region 400bp sequence was obtained using PCR amplification.The method of getting plant genes by microdissecting and microcloning was established,which is particularly useful to obtain a specific gene probe for the further study.