摘要
新鲜牛乳经超速离心,氯仿乙醇处理,丙酮沉淀、透析、DEAE纤维素柱层析等步骤使其中Cu-ZnSOD得到了纯化,提纯后酶的比活为149.5V/mg。该酶对H2O2敏感,并且在272nm和675nm处各有一吸收峰,证明被纯化的酶是Cu-ZnSOD。
A copper/zinc superoxide dismutase from fresh milk was purified to homogeneity by ultracentrifugztion. Chloroform ethanol treatment. acetone sedimention, dialyzation, DEAE-cellulose chromatography. the specific activity of the purified enzyme is 149.5V/mg. The enzyme is sensitire to H 2 O 2 .The enzyme enhibits maximam absortion at 272nm and 675nm. This facts have proved that the enzyme is copper/zinc SOD.