从针叶树营养体组织中提取高纯度DNA的方法

DNA EXTRACTING METHODS FROM VEGETATIVE TISSUE OF CONIFER

ＤＮＡ提取质量是影响ＤＮＡ分析实验结果的关键因素之一，保持不同样本间ＤＮＡ质量的一致性对实验结果的可靠性至关重要，这要求在材料选择和提取方法两个方面都要加以考虑。介绍了从针叶树营养体组织中提取高纯度ＤＮＡ的方法，提取的关键是选择合适的材料，不同于通常采用针叶进行ＤＮＡ提取，本实验选用尚未木质化的针叶树嫩梢进行ＤＮＡ提取，可以在较为简单的实验条件和实验程序下提取高质量ＤＮＡ。按照作者提供的提取方法，可以获得高纯度的，质量较为一致的ＤＮＡ，适用于不同针叶树的ＤＮＡ提取，省时且实验成本较低。

The quality of the isolated DNA is one of the most important facts that influence the result of DNA analysis. Some experiments, such as genomic mapping and population genetics require preparation of large numbers of DNA samples that are correctly identified with respect to the individuals that were the source of the DNA samples. The identity of samples must be maintained, but the samples must be processed quickly and efficiently. Here we describe a simple method to isolate high-quality and identified DNA from vegetative tissue of conifer. The crucial step is to select the suitable material. Different from the common used tissues, whose yielded DNAs are often degraded due to its complicated secondary substance and additive, the tender unwoody terminals are adopted for DNA isoaltion. Here also describes two DNA isolation protocols. According to these protocols, DNA yields and purities were similar for all samples. Highly purified DNA from different conifer species can be obtained following these procedures.