红芪染色体数目及核型分析

Karyotype Analysis of Hedysarun polybotrys Hands.-Mazz

红芪为多年生草本植物。以道地红芪为材料,采用常规压片法制片,结合显微摄影技术,研究了红芪染色体数及其核型,旨在为红芪种质鉴定、起源分析、物种演替、良种培育提供必要的细胞学依据。结果表明,红芪的染色体为二倍体,染色体数目为14,染色体核型公式是2n=14=10m+4sm;其中第4号和第7号染色体为近中着丝粒染色体,其余染色体均为中间着丝粒染色体;核型不对称系数为60.45%,属于"2A"型,是一种原始类型。

The Hedysarun polybotrys Hands.-Mazz. is a perennial herb. No reports have been seen in literature about the karyotype analysis of Hedysarun polybotrys Hands.-Mazz. To study the evolution, identification and fine seeds selection through Hedysarun polybotrys Hands.-Mazz. chromosome number and karyotype, the common （slice-making） method combined with micrograph was used to obtain the chromosome number and karyotype of Hedysarun polybotrys Hands.-Mazz.. The results show that the fukanensis is diploid, and the chromosome number of somatic cells is 2n =14. The karyotype formula is 2n =2x =14 =10m ＋4sm, where the 4th and 7th are submetacentric, and the others are metacentric. Its index of asymmetry is 60.45%. The karyotype of Hedysarun polybotrys Hands.-Mazz is of ＂2A＂ type of stebbins, and it is a primitive species. This study provides a scientific basis for identification of the Hedysarun polybotrys Hands.-Mazz. and Astragalus membranaceus.