玉郎伞提取物对模型大鼠胸膜炎TNF-α、PGE_2、NO的影响

Effects of Millettia pulchra Extracts on TNF-α、PGE_2、NO in Pleuritis Model Rats

目的:研究玉郎伞(YLS)提取物对模型大鼠胸膜炎中肿瘤坏死因子-α(TNF-α)、前列腺素E(2PGE2)和一氧化氮(NO)的影响。方法:实验分为7组,即正常、模型、地塞米松和YLS水提取物(TYLS)高、低剂量及YLS总黄酮(FYLS)高、低剂量组。各组术前连续灌胃7d,于末次给药30min后采用胸膜腔注射角叉菜胶复制胸膜炎模型。复制8h后检测胸腔渗出液体积、渗出液中白细胞计数和TNF-α、PGE2及NO的含量。结果:与模型组比较,TYLS、FYLS高、低剂量组大鼠胸腔渗出液体积、白细胞数量和TNF-α及PGE2含量减少,NO生成无显著改变。结论:YLS提取物对胸膜炎有显著的抑制作用,可能是通过抑制炎性介质PGE2的生成,抑制白细胞浸润和游走及细胞因子TNF-α的升高等环节产生抗炎作用,但与NO的调节无关。

OBJECTIVE： To investigate the effect of Millettia pulchra extracts on tumor necrosis factor α （TNF-α）, prostaglandin E2（PGE2） and nitric oxide （NO） in pleuritis model rats. METHODS： 56 Wistar male rats were randomly divided into control group, model group, dexamethasone （DEX） group, water extract of M.pulchra （TYLS） group （high dose and low dose） and total flavonoids of M.pulchra（FYLS） group （high dose and low dose）. After preoperational intragastric administration for 7 days, the pleuritis model was induced by injecting carrageenan into pleural cavity in 30 minutes after the last medication. The amounts of pleurorrhea, leucocyte, TNF-α, PGE2 and NO in the pleurorrhea were measured at 8 hours after modeling. RESULTS： As compared with the model group, in TYLS and FYLS group the pleurorrhea volume, leukocyte amount, contents of TNF-α and PGE2 reduced markedly, but the synthesis of NO had little change.CONCLUSION：M.pulchra Extracts show a marked inhibitive effect on pleuritis. Their anti-inflammation effects may be related to inhibiting the increase of TNF-α and the infiltration and transmigration of leucocytes, but not associate with the synthesis of NO.