北青龙衣中胡桃苷B的分离及含量测定方法的建立

Isolation of Juglanoside B from the Fresh Rejuvenated Fruits of Juglans Mandshurica and Establishment of Its Content Determination Method

目的从北青龙衣醇提取物中分离胡桃苷B并建立了含量测定的方法。方法利用大孔树脂柱色谱、硅胶柱色谱和反相硅胶柱色谱进行分离纯化,得到胡桃苷B,通过高效液相色谱仪建立了含量测定的方法。结果经核磁共振谱鉴定胡桃苷B为(4S)-4,5-二羟基-α-四氢萘酮-4-O-β-D-吡喃葡糖苷,高效液相色谱洗脱条件为乙腈-水-磷酸(10∶90∶0.2)。结论该方法简便可行、重复性好,胡桃苷B可作为北青龙衣的指标成分建立质量控制的方法。

OBJECTIVE To isolate juglanoside B and establish its content determination method from the fresh rejuvenated fruits of J.mandshurica.METHODS Juglanoside B was isolated by macro-porous resin chromatography,silica gel chromatography and reverse phase silica gel chromatography.The method of its content determination was established by HPLC.RESULTS Juglanoside B was identified as（4S）-4,5-dihydroxy-α-tetralone 4-O-β-D-glucopyranoside on the basis of NMR analysis.Elution condition by HPLC was MeCN-H2O-H3PO4（10：90：0.2）.CONCLUSION The method is simple and has good repeatability.Juglanoside B can be used as indexical component to establish the method of quality control.