摘要
目的从北青龙衣醇提取物中分离胡桃苷B并建立了含量测定的方法。方法利用大孔树脂柱色谱、硅胶柱色谱和反相硅胶柱色谱进行分离纯化,得到胡桃苷B,通过高效液相色谱仪建立了含量测定的方法。结果经核磁共振谱鉴定胡桃苷B为(4S)-4,5-二羟基-α-四氢萘酮-4-O-β-D-吡喃葡糖苷,高效液相色谱洗脱条件为乙腈-水-磷酸(10∶90∶0.2)。结论该方法简便可行、重复性好,胡桃苷B可作为北青龙衣的指标成分建立质量控制的方法。
OBJECTIVE To isolate juglanoside B and establish its content determination method from the fresh rejuvenated fruits of J.mandshurica.METHODS Juglanoside B was isolated by macro-porous resin chromatography,silica gel chromatography and reverse phase silica gel chromatography.The method of its content determination was established by HPLC.RESULTS Juglanoside B was identified as(4S)-4,5-dihydroxy-α-tetralone 4-O-β-D-glucopyranoside on the basis of NMR analysis.Elution condition by HPLC was MeCN-H2O-H3PO4(10:90:0.2).CONCLUSION The method is simple and has good repeatability.Juglanoside B can be used as indexical component to establish the method of quality control.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2010年第1期46-48,共3页
Chinese Journal of Modern Applied Pharmacy
基金
中国博士后科学基金(20060390267)
黑龙江省科技厅青年科技创新基金(QC07C65)
黑龙江省教育厅青年基金(11531279)
哈尔滨市科技局科技创新人才研究专项基金(2008RFQXS106)
关键词
青龙衣
胡桃苷B
含量测定
the fresh rejuvenated fruits of J.mandshurica
juglanoside B
content determination