纺锤芽孢杆菌降解水中萘的特性研究

Study on Biodegradation of Naphthalene by Bacillus fusiformis

从处理石油废水的曝气池污泥中筛选、分离到一株能有效降解萘的菌株,经鉴定为纺锤芽孢杆菌(BFN),研究了其对水中萘的降解特性。结果表明:在温度为30℃、自然pH(6.68～6.76)、接种量为0.2%、(NH4)2SO4浓度为0.15g/L的最适降解条件下,BFN对萘(初始浓度为50mg/L)的降解率在96h内达到99.8%;BFN还具有较好的耐盐度,对高浓度的萘也有较好的耐受性。BFN对萘的降解过程符合一级反应动力学。通过检测不同底物水样的吸光度、pH和底物浓度的变化,发现BFN还能降解苯甲酸、水杨酸、邻苯二甲酸、甲苯、苯酚以及1-萘酚。

A strain of Bacillus fusiformis was isolated from activated sludge of a petroleum wastewater treatment plant and was used for the biodegradation of naphthalene in petroleum wastewater. The optimal experimental conditions are as follows ： temperature is 30℃, pH is 6.68 to 6.76, inoculation rate is 0.2%, and （ NH4） 2SO4 concentration is 0.15 g/L. Under these conditions, the degradation rate of naphthalene reaches up to 99.8% within 96 h when the initial naphthalene concentration is 50 mg/L. In addition, the strain is able to tolerate salinity and high concentration of naphthalene. The naphthalene biodegradation process follows the first-order reaction kinetics. Furthermore, the strain＇ s capability of degrading benzoic acid, salicylic acid, o-phthalic acid, toluene, phenol and 1-naphthol is shown by measuring the absorbance, pH and degradation of substrates during the treatment of different substrates.